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   Molecular and Human Genetics Department in Varanasi, Molecular and Human Genetics Department in Banaras Hindu University Varanasi, Molecular and Human Genetics Department in Uttar Pradesh, Immunology, Immunogenetics, Systemic Lupus Erythematosus, SLE, Netosis, 
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   we are involved in development of monoclonal Abs and biosimilars and our efforts to manage Dengue fever through immunomodulation have resulted in a novel formulation" />


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				 	<h3>Geeta Rai, Ph.D, MNASI, FSAB</h3>
				 	<p><strong>Associate Professor</strong><br />
                    <strong>Department of Molecular & Human Genetics</strong><br />
                     <strong>Institute of Science</strong><br />
                   <strong>Banaras Hindu University,Varanasi- 221005 (India)</strong></p>
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                      <h3>Welcome to the Immunogenetics laboratory of Geeta Rai, PhD.</h3>
                      <p>Our current research program comprises of several independent but synergistically interacting projects with major focus in three domains:</p>
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                         <li><i class="fa fa-star"></i><span>Autoimmune disease Systemic Lupus Erythematosus (SLE).</span></li>
                          <li><i class="fa fa-star"></i><span>Defective mechanisms of the innate immunity in low birth weight (LBW) newborns. </span></li>
                           <li><i class="fa fa-star"></i><span>Stem cell studies to develop better tools for regenerative medicine.</span></li>
                          <li><i class="fa fa-star"></i><span>Biosimilar/Monoclonal Antibodies development</span></li> 
                      
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                         <li><i>1.</i><span>My research contribution in SLE initiates from development of a novel disease model and extends to identification, optimization and evaluation of disease processes aimed at amelioration of the disease state in human beings. The salient research outputs have been, elucidation of role of Toll like Receptors in paradigm of diverse clinical manifestations in SLE (Patent, 2013(a)., best oral presentation award, Immunocon 2012).  Significant efforts have led to successful characterization of novel markers in SLE using genomic and proteomic approaches. Results of miRNA and global transcriptome sequencing studies have helped provide a strong basis for dintinguishing SLE in 3 distinct subgroups with a potential of impacting treatment modality.</span></li>
                          <li><i>2.</i><span>Transcriptomic and proteomic studies involving newborns have resulted in identification of defective immune pathways responsible for increased susceptibility of LBW newborns towards life threatening infections and mortality (GP Talwar young scientist award, Immunocon 2013). Our observations on impaired Neutrophil Extra trap (NET) formation in LBW newborns (Best oral presentation award, Immunocon 2013’) led to development of novel formulation to improve immune efficiency and NET formation (Patent 2013(b)).</span></li>
                           <li><i>3.</i><span>Our recent research focus in stem cells, has identified defective differentiation of cord blood hematopoetic stem cells in specific group of newborns.</span></li>
                            <li><i>4.</i><span>Development of Biosimilars for various Immunopathologies.</span></li><br />
                        <li><span>In addition, we are involved in development of monoclonal Abs and biosimilars and our efforts to manage Dengue fever through immunomodulation have resulted in a novel formulation (Patent 2016). Development of biogenic silver nanoparticles which target and destroy cancer cells has been our most recent research effort (Patent 2017).</span></li>
                      
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                   	<p class="text-justify"><strong><i class="fa fa-star"></i></strong><strong>Post Doctoral Fellow</strong><i class="glyphicon glyphicon-arrow-right"></i>Lab of Immunology, NIAID, National Institute of Health, Bethesda, USA 2003-2006.</p>
				 	
				 	<p class="text-justify"><strong><i class="fa fa-star"></i></strong><strong>Ph.D.</strong> (Immunology)<i class="glyphicon glyphicon-arrow-right"></i>Sanjay Gandhi Post Graduate Institute of Medical Sciences (SGPGIMS), Lucknow, India (2002). </p>
                    <p class="text-justify"><strong><i class="fa fa-star"></i></strong><strong>Master of Science</strong> (Biochemistry and Biotechnology)<i class="glyphicon glyphicon-arrow-right"></i>Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, India (1997). </p>
                    
                    <p class="text-justify"><strong><i class="fa fa-star"></i></strong><strong>Bachelor of Science</strong> (Botany, Zoology, Chemistry)<i class="glyphicon glyphicon-arrow-right"></i>Banaras Hindu University, Varanasi, India (1995). </p>
				
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				 	<p class="text-justify"><strong><i class="fa fa-star"></i></strong><strong>G.P. Talwar Young scientist award(Immunocon 2013)<i class="glyphicon glyphicon-arrow-right"></i></strong>Singh VV, Chauhan SK, Rai R, Soni UK, Ashok Kumar, Rai G. Impaired Genetic Mechanisms of Innate Immunity in Low Birth Weight Newborns. 40th Ann. Conf. of Indian Immunology Society, University of Delhi, North Campus, New Delhi, November 15 -17, 2013; P-30. </p>
                    <p class="text-justify"><strong><i class="fa fa-star"></i></strong><strong>Firstprize in oral presentation (Immunocon 2013)<i class="glyphicon glyphicon-arrow-right"></i></strong>	Soni UK, Singh VV, Chauhan SK, Rai R, Ashok Kumar, Rai G. Leukotriene B4 receptor regulates Neutrophil Extracellular Trap formation by activation of Gas/Rap1/b-Raf/ERK1/2 signaling pathway. 40th Ann. Conf. of Indian Immunology Society, University of Delhi, North Campus, New Delhi, November 15 -17, 2013; P-60. </p>
                    <p class="text-justify"><strong><i class="fa fa-star"></i></strong><strong>Firstprize in oral presentation  (Immunocon 2012)<i class="glyphicon glyphicon-arrow-right"></i></strong>	Chauhan SK, Singh VV, Rai R, Rai M, Rai G. TLR7 and TLR9 upregulation associates    with autoantibodies against specific nuclear targets in systemic lupus erythematosus. 39th Ann. Conf. of Indian Immunology Society, Banaras Hindu University, Varanasi, November 9-11, 2012; P-40. </p>
                    <p class="text-justify"><strong><i class="fa fa-star"></i></strong><strong>Secondprize in oral presentation (Immunocon 2012)<i class="glyphicon glyphicon-arrow-right"></i></strong>	Soni UK, Singh VV, Chauhan SK, Rai R, Kumar A, Rai G. HL60 differentiation: Highest ROS activity with maximum survivability of neutrophil-like cells achieved after 120 hrs by All Trans Retinoic Acid (ATRA) induction. 39th Ann. Conf. of Indian Immunol.Soc., BHU, Varanasi, November 9-11, 2012; P-59. </p>
                    
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							  <h3>Systemic Lupus Erythematosus</h3>
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						      <p class="text-justify">SLE(Systemic Lupus Erythematosus) is a complex, chronic autoimmune disease with significant mortality and morbidity. It is characterized by a diverse range of autoantibodies against nuclear/ cytoplasmic components.<span class="text-justify moretext" style="display:none;">The clinical manifestations of SLE are highly variable with multiple organs and organ systems affected; these include skin joints, heart, kidney, and the nervous. Presence of autoantibodies, to RNA spliceosomalribonucleoproteins and dsDNA are characteristic in this disease. We in our lab identified differential Toll-like receptors (TLRs) expression in different subsets of SLE patients based on autoantibodies present in their sera . In a rabbit model of neuropsychiatric lupus, we have also established a link between different autoantibody repertoires with differential gene expression in SLE. We aim to understand different genetic and epigenetic regulatory mechanisms operative in different SLE subsets. Our goal is to uncover the molecular basis for such tremendous heterogeneity and differential response to the therapies observed in SLE patients. Further, the identification of these biomarkers which can  help the clinicians in characterizing the patients into appropriate groups and planning a more targeted treatment strategy.</span>  </p>
                      
                        
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							  <h3>Immunity in Neonates</h3>
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						      <p class="text-justify">Due to immuno-compromised status of low birth weight (LBW) newborns and inefficient immune responses they are highly susceptible to various infections resulting into sepsis, and have high morbidity and mortality rates.<span class="text-justify moretext" style="display:none;">Netosis is a unique antimicrobial function of the neutrophils in which neutrophil cells are activated in response to pathogenic infection, and release their chromatin outside the cell membrane along with antimicrobial peptides such as neutrophil elastase, Myeloperoxidase, Defensin 1 and 2, Cathepsin G. This histone mixed DNA make a net-like structure termed as NET which is employed in trapping and killing of the pathogens. In our earlier studies we have found poor NETs formation in LBW newborns.</span>   </p>
                         
                                
                                <p class="text-justify moretext" style="display:none;">Our aim is to investigate novel genes and protein molecules not known earlier to be involved in netosis which is a marker of innate immunity in newborns and toboost up the NETosis in LBW newborns to protect them from infection.</p>
                             
                              
                          
                          
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						      <p class="text-justify">Cord blood is conventionally known to contain stem cells that are easily accessible at birth and also capable of differentiating into various cell types and therefore considered as relevant source of stem cells especially for the treatment of neonatal diseases.   </p>
                          
                           

                   
                                <p class="text-justify moretext" style="display:none;">Our laboratory has been working on understanding the role of immunological responses in outcome of life threatening infections in low birth weight (LBW) newborns (birth weight < 2.5 Kg) for the past few years. The LBW newborns have long been known to have significantly lesser number of immune cells makes them highly susceptible for microbial infections. Since all blood cells are generated through hematopoiesis. This prompted us to focus our investigation on evaluating hemotopoeisis and differentiation occurring at the hematopoietic stem cell level in these newborns. Our stem cell research will eventually make great contributions to the development of safe, efficacious and a novel therapeutic intervention against life threatening microbial infections and resulting mortality.</p>
                                
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